Headquartered in Carlsbad, California, and with offices in Boston, Massachusetts, and Dublin, Ireland, Ionis has been at work for more than three decades discovering medical breakthroughs that have redefined life for people with serious diseases. We are pioneers in RNA-targeted medicines, and our platform continues to revolutionize drug discovery and transform lives for patients with unmet needs.  With five currently marketed medicines for serious diseases and an expansive late-stage pipeline, we continue to build upon our groundbreaking innovations in science and technology to provide greater value to patients; and we are well positioned financially to deliver on our strategic goals.

At Ionis, we pride ourselves on cultivating a challenging, motivating, and rewarding environment that fosters innovation and scientific excellence. We know our success is a direct result of the exceptional talent and dedication of our employees.

With an unprecedented opportunity to change the course of human health, we look to add diverse individuals, skill sets, and perspectives to our exceptional team. We continue to invest time, money, and energy into making our onsite and remote work environments a place where solid and lasting relationships are built and where our culture and employees can thrive.

We are building on our rich history, and believe our greatest achievements are ahead of us.  We invite you to apply and join us if you’re passionate about the opportunity to have a meaningful impact on patients in need, our employees, and our organization.  Experience and contribute to our unique culture while you develop and expand your career!

In addition, the internship program allows students to grow both professionally and personally through interactions with research and business professionals in support of projects aligned with department initiatives. Students will learn and work under a practicing professional who will provide guidance and mentorship.

Gene Editing (Chemistry)

Summary:

We are seeking a highly motivated intern to join our gene-editing team focused on the chemical synthesis of modified nucleosides and oligonucleotides. The intern will participate in synthesizing the appropriate chemical building blocks for solid-phase oligonucleotide synthesis and post-synthetic reactions, as well as the purification and characterization of synthetic guide RNAs to support our research in CRISPR focused gene-editing. The ideal candidate will have an understanding of basic chemistry terms and principles in addition to the ability to work collaboratively in a fast-paced environment.

Intern Responsibilities:

Nucleoside/Oligonucleotide Synthesis: Synthesis, purification and characterization of modified phosphoramidite and other small molecule building blocks. Use of automated synthesizers to produce RNA oligonucleotides. Develop in-house capping methodologies for efficient addition of post-synthetic oligonucleotide modifications. Purify oligonucleotides using various techniques such as HPLC, PAGE, or other relevant methods. Use biochemical assays to determine reaction efficiencies and the effects of modifications on custom oligonucleotides. Research and Development: Participate in the development of new chemical modalities for CRISPR guide RNAs. Participate in weekly department meetings. Maintain accurate and detailed laboratory records in accordance with company policies and regulatory requirements. Communicate findings and progress to project leaders.

Requirements:

Currently enrolled in a chemistry/biochemistry undergraduate/graduate program in the United States. Knowledge of basic organic chemistry techniques. Hands on chemistry lab experience is a bonus. Experience in one or more of the following: Synthesis, isolation, purification, and analysis of modified oligonucleotides. Synthesis of custom nucleosides and related small molecules. Utilization of biochemical reactions, enzymatic reactions, and biochemical assays. Experience working with HPLC/FPLC systems, RNA, gel electrophoresis, or analyzing RNA secondary structures a plus. Excellent problem-solving and analytical skills. Strong communication and interpersonal skills. Ability to work independently and collaboratively in a team environment.

The student intern will be directly integrated into the core chemistry team of the gene-editing program at Ionis Pharmaceuticals. This will include participating in weekly meetings where the student will present the progress of their research. In addition, the intern will participate in the chemical synthesis of nucleic acid building blocks using organic chemistry followed by their characterization using spectroscopic techniques. These building blocks will be further incorporated into synthetic oligonucleotides for use in in vitro biological and biochemical assays. Additionally, the intern will be responsible for proper laboratory practices including lab notebook upkeep and reagent preparation.

Additional Requirements:

MUST be currently enrolled in a U.S. Undergraduate Program and not graduating prior to the completion of the Ionis internship. MUST be able to begin internship between 6/9 and 6/16/2025 MUST be able and willing to remain in the program through at least 8/15/2025 MUST be able and willing to commit to a minimum 40-hour workweek and work onsite in Carlsbad, CA. Housing and travel assistance not provided for—local applicants with suitable transportation are preferred

Program Highlights: 

The program is designed to complement a student’s undergraduate studies with relevant, hands-on industry experience Ionis offers paid positions Weekly stipend (for onsite interns only) Professional Networking Fun Group Outings End-of-program project presentations Networking and bonding social events Interaction with diverse and motivated teams/departments

 

Please visit our website, http://www.ionis.com for more information about Ionis and to apply for this position

The pay scale for this position is $18 to $23 per hour.

NO PHONE CALLS PLEASE. PRINCIPALS ONLY. APPLICATION DEADLINE IS April 30, 2025. PLEASE INCLUDE A COVER LETTER RELEVANT TO THE POSITION APPLYING FOR. THE EVALUATION PROCESS MAY COMMENCE PRIOR TO THIS DEADLINE. 

Ionis Pharmaceuticals, Inc. and all its subsidiaries are proud to be EEO employers.