POSITION SUMMARY:

Under the direction of the manager, this position is responsible for the production of cell-based products including but not limited to: hybridoma antibodies and recombinant antibodies. Primarily responsible for cell culture and managing multiple purifications systems.

DUTIES AND RESPONSIBILITIES: 

• Manipulate multiple cell lines (both adherent and suspension), such as mouse hybridoma cell , Expi 293 and Expi CHO cells for the production of monoclonal antibodies and other cell based products.

• Execute transfections for the production of recombinant QRM antibodies.

• Use the following cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer.

• Use AKTA purification systems

• Provide general cell culture laboratory maintenance. Keep process areas stocked with essential equipment and supplies and maintain overall laboratory appearance and cleanliness.

• Coordinate regular equipment maintenance and calibrations; complete and document equipment validation as required in MFG.

• Perform daily scheduled cell culture and purification processes to meet production demand

• Perform vialing and storage of cell based products.

• Maintain and organize inventory of supplies, including but not limited to cell stocks and records, to support daily production.

• Other duties as assigned.

EXPERIENCE AND QUALIFICATIONS:

• Bachelor's degree in Biology, Chemistry, Biotechnology, Engineering or a related field or equivalent applicable work experience. May substitute degree for equivalent combination of experience/education.

• Minimum of three months of experience performing cell culture using aseptic technique in a laboratory setting required.

• Experience in a GMP/GLP, ISO Certified environment preferred.

KNOWLEDGE, SKILLS AND ABILITIES:

• Knowledge of manufacturing mammalian cell based products preferred 

• Knowledge of Hybridoma cell culture and expansion preferred.

• Knowledge of common cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer.

• Proficiency in MS Office Suite and SharePoint preferred.

• Attention to detail with demonstrated commitment to excellence and performance 

• Organizational, planning, and time management skills with the ability to manage multiple and often changing priorities while meeting time-sensitive deadlines and deliverables.

• Good communication skills with the ability to organize, present, and articulate ideas both verbally and in writing.

• Basic knowledge of aseptic technique; ability to complete cell culture in the absence of antibiotics.

• Ability to work both independently and as an effective team member.

• Willingness and ability to acquire new skills in various cell culture methods as necessary.

• Ability to understand and follow safety procedures.

• Ability to move/lift up to 40 pounds.

PHYSICAL DEMANDS:

• While performing the duties of this job, the individual is frequently required to sit and stand for long periods. The individual is occasionally required to walk; use hands and fingers to operate, handle, or feel objects, tools, or controls; reach with hands and arms.

• May be required to move/lift up to 40 pounds.

WORK ENVIRONMENT:

• This position is performed in a traditional industrial laboratory environment but can also include work in a laboratory environment (which can include work with chemicals and hazardous materials.