Pittsburgh, Pennsylvania, USA
3 days ago
Laboratory Research Technician - Moatti lab

Required Education and Experience

Appropriate BSC in Cell Biology, Molecular Biology, Biomedical Engineering or a related field.

Required Knowledge, Skills, and Abilities

Cell biology skills:

Cell culture 2D 3D

Animal skills:

Comfortable with Handling animalsComfortable with IP/IV injection Comfortable with Survival surgery

Excellent written and oral communication skills

Ability to work independently and collaboratively

Preferred skills:

Molecular biology skills:

RNA extractionPCRRNA sequencingExtracellular vesiclescAMP assayIP3 assayMass spectrometryPlasmid preparationStem CellsMetabolomics Data analysisOrgan of Corti dissectionPerilymph samplingImage processingLeadership and project management skills

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Cell cultureliposome modificationsextracellular vesicles isolation and loadingHistology and tissue processing including fixation (perfusion and immersion)inner ear gross and fine dissectiontissue clearringsectioningimmunohistochemistry and fluorescent imagingAccurate and detailed record keeping in electronic lab notebooks.In vivo delivery to the inner ear of pigs which might involve using adeno-associated viral vectors and nonviral vectorsAssays of physiological (auditory brainstem response) and behavioral thresholds for hearing function before and after herapy in animal models.RNA SequencingMass spectrometryprotein purificationHPLCHandling human and animal samples
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