Position is currently available for a Laboratory Specialist within the Vascular Medicine Institute of the University of Pittsburgh, Department of Medicine. The ideal candidate should have extensive experience with management of mouse colony, mouse genotyping using PCR and gel electrophoresis. Experience with standard molecular biology and biochemistry techniques is highly desired including Western Blot, DNA-gel electrophoresis, RT-PCR, immunohistochemistry, co-immunoprecipitation, ELISA, cell transfection, etc. Prior work with mouse models of disease and genotyping of mice is preferred. Experience in performing tail vein and IV injections in mice are highly desirable. Excellent verbal and written skills, attention to detail, and precise record keeping is expected. Cell culture experience, including human and rodent endothelial cells, is highly appreciated. Duties include management of mouse colony and genotyping, running biochemical assays, ordering lab supplies, and record keeping.

Main Duties:

Manage mice colonies, breeding according to investigators’ needs, caring for animals, and maintaining their health and well-being.

Perform wide variety of biomolecular assays including western blot, PCR, immunoprecipitation, cell culture, ELISA, and immunoassay if needed.

Perform complex animal procedures including tail snips and blood collection for genotyping and other more advanced genotyping techniques.

Provide weekly reports on size and status of each colony, assisting in reconciling animal-related accounts, and other duties as assigned.

Ordering and management of lab supplies including reagents and mice. Also management of IACUC protocols including modification and submission of new protocols.

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

Process data and use computer keyboard. Accurate visual recognition of small samples and repetitive pipetting; handling mice requires accurate eye to hand coordination, excellent visual acuity, and manual dexterity. Must be very organized and be able to keep a database for mouse colonies, biological specimens, reagents, chemicals, etc. Must be able to reach/stack consumables on shelves.