Laboratory Technician II-Department of Neurosurgery-Preston A. Wells, Jr. Center for Brain Tumor Immunotherapy Apply now (https://secure.dc4.pageuppeople.com/apply/674/gateway/default.aspx?c=apply&lJobID=534288&lJobSourceTypeID=796&sLanguage=en-us) Job no: 534288 Work type: Staff Full-Time Location: Main Campus (Gainesville, FL) Categories: Health Care Administration/Support Department: 29190000 - MD-NEUROLOGICAL SURGERY Classification Title: Laboratory Technician II Job Description: Under the direction of the Principal Investigator (PI), assist with basic surgical procedures on laboratory animals. Basic procedures will include developing T cell based immunotherapies and other vaccines, animal behavior such as rotarod, gait etc (according to IACUC protocols). This involves dendritic cell preparations, tail vein injections, and the ability to administer anesthesia as well as SQ, IV, IP and IM injections to animals, and performing perfusions. Under the supervision of the PI, analyze tissues using immunohistochemistry, fluorescent microscopy, and confocal microscopy as necessary. Perform ELISAs or Luminex assays as appropriate to quantify proteins of interest and analyze data. Perform DNA and RNA extractions as necessary for generating the vaccines. Assess results and modify techniques as needed with PI approval. Research PubMed articles. Ensure that experiments are completed in a timely manner. Analyze data, prepare written reports and presentations of research findings as necessary. Assist in the maintenance of a database of all research results. Retrieve and manipulate computerized data to be used in publications and presentations. Obtain, analyze and store digital images acquired during immunohistochemistry, fluorescent microscopy, confocal microscopy and anatomical images acquired post-operatively. Maintain computer databases and a daily log of research activities/results. Maintain tissue bank. Coordinate general laboratory maintenance and work flow, include ordering supplies, maintaining inventory, assisting with budgeting of laboratory funds, and maintaining compliance with EH&S. Train, direct and coordinate activities of undergraduate assistants, volunteers and other laboratory personnel. Write Standards of Procedure (SOPs) and protocol addenda. Ensure compliance with IACUC, EH&S and radiation control. Expected Salary: $19.32-$21.89 Minimum Requirements: Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college coursework or vocational/technical training may substitute at an equivalent rate for the required experience. Preferred Qualifications: Two or more years of experience in laboratory with small animal experiment is preferred. Special Instructions to Applicants: For consideration, please complete an application and include a cover letter, resume and the names and email addresses of three to five references. The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training. To claim veterans' preference, indicate the claim on the application and provide required documentation by uploading a DD-214 (or equivalent certification from the Department of Veteran Affairs) and any additional supporting documentation based on the type of claim. Application must be submitted by 11:55 p.m. (ET) of the posting end date. Health Assessment Required:No Advertised: 18 Dec 2024 Eastern Standard Time Applications close: 05 Jan 2025 Eastern Standard Time