The laboratory of Dr. Andreas Tolias is seeking a talented LSRP1 to join a multi-disciplinary research team aimed at understanding the cellular diversity of brain circuits. The specific goals of these positions will be to use whole-cell patching methods, single-cell RNA-sequencing and immunochemistry to probe morphology, electrophysiology and transcriptome of the individual neurons of brain circuits across cortical areas and species including humans. In particular, Patch-seq, a method we recently developed, that combines whole-cell patch-clamp recordings and single-cell RNA sequencing (scRNA-seq). The individual will receive extensive one-on-one training with postdocs and junior faculty on cellular electrophysiology to develop the necessary skills to acquire high-quality data. They will also have opportunity of learn in vivo multi-photon imaging and multi-electrode recordings using Neuropixels and advance their computational and programming skills.
The ideal applicants should have some experience in cellular electrophysiology, PCR or RNA-sequencing. The candidates without these experiences will be also considered if they are willing to commit the efforts to develop these skills.
Duties Include:
·Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
·Independently conduct experiments; maintain detailed records of experiments and outcomes.
·Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
·Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
·Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
·Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
·Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned
DESIRED QUALIFICATIONS:
Master’s degree in a basic science. One year of directly related experience with patch-clamp
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
·General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
·Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
·Ability to maintain detailed records of experiments and outcomes.
·General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
·Ability to work under deadlines with general guidance.
·Excellent organizational skills and demonstrated ability to accurately complete detailed work.
CERTIFICATIONS & LICENSES:
None
PHYSICAL REQUIREMENTS*:
·Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
·Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
·Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
·Must have correctible vision to perform duties of the job.
·Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
·Ability to lift heavy objects weighing up to 50 pounds.
·Ability to work in a dusty, dirty, and odorous environment.
·Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
WORKING CONDITIONS:
·May require working in close proximity to blood borne pathogens.
·May require work in an environment where animals are used for teaching and research.
·Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
·Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
·May require extended or unusual work hours based on research requirements and business needs.
The expected pay range for this position is $31.40 to $38.89 per hour. Stanford University provides pay ranges representing its good faith estimate of what the university reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location and external market pay for comparable jobs.
At Stanford University, base pay represents only one aspect of the comprehensive rewards package. The Cardinal at Work website (https://cardinalatwork.stanford.edu/benefits-rewards) provides detailed information on Stanford’s extensive range of benefits and rewards offered to employees. Specifics about the rewards package for this position may be discussed during the hiring process.