**Job Description** Title: Postdoc in Shi lab Salary: $ 73,55 Department: Pharmacological Sciences Physical work location: (must be proper street address): Name PI or Supervisor: (include phone and email): Yi Shi wally.yis@gmail.com Web link to Lab: www.shi-lab.org Web link to Department: https://icahn.mssm.edu/about/departments/pharmacological-sciences Administrative Contact: (phone and email): Yoori Kim, 212-6598651, yoori.kim@mssm.edu **Qualifications** Experience Required: A Ph.D. in biochemistry, bioengineering, immunology, chemical biology, structure biology or a related field. Strong expertise in protein/antibody engineering or chemical biology. Hands-on experience in biochemistry and biophysics. Experience in cell-based assays, live-cell imaging, and immunoassays. Familiarity with chemical conjugation techniques for targeted payload delivery is desirable. Computational skills in protein modeling, docking, or protein design are a plus. A strong track record of peer-reviewed publications in relevant fields. Ability to work independently and collaboratively in a dynamic research environment. , 858 - Pharmacological Sciences - ISM, Icahn School of Medicine **EOE Minorities/Women/Disabled/Veterans** The Mount Sinai Health System is an equal opportunity employer. We comply with applicable Federal civil rights laws and does not discriminate, exclude, or treat people differently on the basis of race, color, national origin, age, religion, disability, sex, sexual orientation, gender identity, or gender expression. We are passionately committed to addressing racism and its effects on our faculty, staff, students, trainees, patients, visitors, and the communities we serve. Our goal is for Mount Sinai to become an anti-racist health care and learning institution that intentionally addresses structural racism.? **Responsibilities** Details of Research Project: The incumbent is expected to lead one or more innovative research projects in protein engineering and therapeutics, with a primary focus on nanobody development for next-generation therapeutic and diagnostic platforms. This role involves designing and optimizing nanobody-based solutions for a range of biomedical applications, leveraging cutting-edge AI-driven approaches, structural biology, and bioengineering techniques. Projects include: 1) Apply AI-driven design and engineering strategies to develop nanobody candidates targeting infectious diseases, cancer, diabetes, and neurodegenerative disorders. 2) Develop biosensor platforms for high-sensitivity detection of blood biomarkers. 3) Engineer and optimize multispecific and multivalent nanobodies for enhanced antiviral and immunotherapy applications. 4) Develop novel strategies to facilitate membrane target degradation and payload delivery of chemical probes and inhibitors. Collaborative Research & Development: Work closely with cross-disciplinary teams in structural biology, computational modeling, immunotherapy, and chemical biology, to advance the development of next-generation theradiagnostic technologies. The incumbent will also contribute to manuscript preparation, grant proposals, and presentations to support research funding and dissemination of findings. Technical Duties: The incumbent will be responsible for executing advanced research protocols related to nanobody engineering, therapeutic development, and biosensor technology. Key technical duties include: Protein/antibody Engineering: Protein Expression & Purification: Expression of recombinant nanobodies and fusion constructs in bacterial, mammalian, and yeast systems. Purification using affinity chromatography and size-exclusion chromatography. Protein quality assessment through SDS-PAGE, Western blot, and biophysical and binding affinity characterization (DSF, DLS, MST, SPR, BLI, etc.). Chemical conjugation of nanobodies with payloads (small molecules, toxins, fluorescent probes). Cellular & Functional Assays: Conducting cell-based binding and internalization assays using flow cytometry and confocal microscopy. Functional testing of multivalent nanobody constructs in immunotherapy applications including cell-based assays and potentially animal models. Educational and other Requirements for the position: PhD **Goals/Outcomes of the Research Project:** The research project aims to develop and translate next-generation nanobody-based therapeutics and diagnostic platforms for applications in infectious diseases, cancer, neurodegenerative disorders, and metabolic diseases. The incumbent will play a key role in advancing protein therapeutics and diagnostic tools, contributing to innovations with real-world impact. The successful candidate is expected to publish high-quality papers, contribute to patent applications, and drive forward key discoveries that support both academic and translation. Additionally, we are committed to mentoring and supporting the candidate’s career development, providing opportunities to transition into leading roles in academia, biotechnology, or pharmaceutical industries. **About Us** **Strength Through Diversity** The Mount Sinai Health System believes that diversity, equity, and inclusion are key drivers for excellence. We share a common devotion to delivering exceptional patient care. When you join us, you become a part of Mount Sinai’s unrivaled record of achievement, education, and advancement as we revolutionize medicine together. We invite you to participate actively as a part of the Mount Sinai Health System team by: + Using a lens of equity in all aspects of patient care delivery, education, and research to promote policies and practices to allow opportunities for all to thrive and reach their potential. + Serving as a role model confronting racist, sexist, or other inappropriate actions by speaking up, challenging exclusionary organizational practices, and standing side-by-side in support of colleagues who experience discrimination. + Inspiring and fostering an environment of anti-racist behaviors among and between departments and co-workers. At Mount Sinai, our leaders strive to learn, empower others, and embrace change to further advance equity and improve the well-being of staff, patients, and the organization. We expect our leaders to embrace anti-racism, create a collaborative and respectful environment, and constructively disrupt the status quo to improve the system and enhance care for our patients. We work hard to create an inclusive, welcoming and nurturing work environment where all feel they are valued, belong and are able to advance professionally. Explore more about this opportunity and how you can help us write a new chapter in our history! **“About the Mount Sinai Health System:** Mount Sinai Health System is one of the largest academic medical systems in the New York metro area, with more than 43,000 employees working across eight hospitals, more than 400 outpatient practices, more than 300 labs, a school of nursing, and a leading school of medicine and graduate education. Mount Sinai advances health for all people, everywhere, by taking on the most complex health care challenges of our time — discovering and applying new scientific learning and knowledge; developing safer, more effective treatments; educating the next generation of medical leaders and innovators; and supporting local communities by delivering high-quality care to all who need it. Through the integration of its hospitals, labs, and schools, Mount Sinai offers comprehensive health care solutions from birth through geriatrics, leveraging innovative approaches such as artificial intelligence and informatics while keeping patients’ medical and emotional needs at the center of all treatment. The Health System includes approximately 7,400 primary and specialty care physicians; 13 joint-venture outpatient surgery centers throughout the five boroughs of New York City, Westchester, Long Island, and Florida; and more than 30 affiliated community health centers. We are consistently ranked by U.S. News & World Report's Best Hospitals, receiving high "Honor Roll" status, and are highly ranked: No. 1 in Geriatrics and top 20 in Cardiology/Heart Surgery, Diabetes/Endocrinology, Gastroenterology/GI Surgery, Neurology/Neurosurgery, Orthopedics, Pulmonology/Lung Surgery, Rehabilitation, and Urology. New York Eye and Ear Infirmary of Mount Sinai is ranked No. 12 in Ophthalmology. U.S. News & World Report’s “Best Children’s Hospitals” ranks Mount Sinai Kravis Children's Hospital among the country’s best in several pediatric specialties. The Icahn School of Medicine at Mount Sinai is ranked No. 14 nationwide in National Institutes of Health funding and in the 99th percentile in research dollars per investigator according to the Association of American Medical Colleges. Newsweek’s “The World’s Best Smart Hospitals” ranks The Mount Sinai Hospital as No. 1 in New York and in the top five globally, and Mount Sinai Morningside in the top 20 globally. The Mount Sinai Health System is an equal opportunity employer. We comply with applicable Federal civil rights laws and does not discriminate, exclude, or treat people differently on the basis of race, color, national origin, age, religion, disability, sex, sexual orientation, gender identity, or gender expression. We are passionately committed to addressing racism and its effects on our faculty, staff, students, trainees, patients, visitors, and the communities we serve. Our goal is for Mount Sinai to become an anti-racist health care and learning institution that intentionally addresses structural racism.” **EOE Minorities/Women/Disabled/Veterans** **Compensation Statement** Mount Sinai Health System (MSHS) provides a salary range to comply with the New York City Law on Salary Transparency in Job Advertisements. The salary range for the role is $72500 - $80000 Annually. Actual salaries depend on a variety of factors, including experience, education, and hospital need. The salary range or contractual rate listed does not include bonuses/incentive, differential pay or other forms of compensation or benefits.