The Mansoor lab seeks a postdoctoral scholar who is interested in structural biology and drug development. The scholar will receive training in the expression and purification of membrane proteins for biophysical and structural analysis, specifically single particle cryogenic electron microscopy (Cryo-EM), as well as training in electrophysiology.
Applicants must be NIH training grant eligible (US Citizen or Green Card holder).
Applicants should send a cover letter describing research experience and interests, a CV, and contacts for references to Steven E. Mansoor (mansoors@ohsu.edu).
The Mansoor Lab studies the molecular structure and function of ion channels and receptors in the cardiovascular system to develop novel small molecules for the treatment of conditions such as hypertension, angina, and platelet aggregation. Typical techniques and experiments in the lab include, but are not limited to, molecular biology, tissue culture, electrophysiology experiments in both Xenopus oocytes and mammalian cells, and protein expression and purification preparations to get isolated receptor for structural studies, including both X-ray crystallography as well as single particle cryogenic electron microscopy. Applicants must have prior biochemistry and molecular biology experience and be comfortable with these types of experiments. Preference will be given to applicants with previous training in membrane protein biochemistry, X-ray crystallography and/or cryogenic electron microscopy.
Function/Duties of Position Primary Research (including planning, granting, execution, analysis, publication)a. Maintain laboratory compliance documentation for human, animal, biological and radiological work.b. Be familiar with lab safety infrastructure and ensure compliance with all safety regulations.
c. Interact with biosafety, chemical safety, and safety officers on behalf of the Principal Investigator.a. Generate data and research analysis for investigator's use in preparation of technical reports and publications.
b. Assist the PI with manuscript preparation and grant applications.
c. Present, or assist in the presentation of, research projects at retreats, seminars, conferences and other OHSU or public venues.Providing training to users on lab practices and protocols, including sample preparation and instrumentation use.Maintain an accurate and detailed laboratory notebook of all experiments performed. Participate in lab meeting.Training of lab personnel and supervision of Research Assistants, students and other lab members.Perform routine lab operations, including but not limited to the purchase of laboratory supplies, monitoring supplies and maintaining all laboratory equipment.Other responsibilities as assigned by the supervisor in furtherance of the lab’s research and the university’s missions and goals of teaching, research, patient care, outreach and public service. Required Qualifications PhD in biological sciences or a related fieldExperimental design and interpretation of resultsBasic molecular biological methods of DNA manipulation and analysisGene analysis techniquesBasic protein analysis techniques such as western blots, immunoprecipitation and immunostaining skills, mammalian cell cultureStrong organizational and interpersonal skillsStrong scientific curiosity, problem-solving skills and research integrityHigh attention to detail and accuracyDemonstrated success working independently and as a member of a collaborative teamAbility to communicate effectively, in both written and oral formatsWorking knowledge of office computer applications, such as Word, Excel and PowerPoint Preferred Qualifications PhD in Biochemistry or Biophysics/PhysicsExperience identifying, manipulating, or using nucleic acid aptamers, ideally from de novo selection by SELEX
SELEX, nucleic acid and protein purification, chromatography, biophysical binding analysis, analytical biochemistry
Programming using Unix
Knowledge of membrane protein biochemistry.
Additional DetailsPosition is located at the OHSU Marquam Hill campus in Portland, Oregon. The work schedule will be set by the supervising faculty, dictated by the needs of the research and planned experiments. Working evenings, weekends and extended hours may be required depending on the needs of the research. Work environment is in an open wet lab, in the presence of laboratory chemicals and automated equipment.
Employee will need to work at lab benches, desks and/or microscopes for up to 8 hours at a time. Ability to perform repetitive lab manipulations (gripping, grasping, reaching, twisting), move lab equipment up to 50 lbs with accommodation, perform image analysis operation and help others with equipment use. Personal protective equipment must be worn as required. (Gloves, mask/respirator, eyewear, lab coat, etc.) Willing to work with radioactive materials, recombinant viruses, and possibly small animals such as mice and rats.
Must be able to communicate orally and in writing the results of scientific experiments; must be able to use a standard computer and screen up to 6 hours per day.
All are welcome Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.