The overall focus of the Charles lab is to decrease the global burden of mucosal and enteric infections of import affecting resource-poor and marginalized populations. More specifically, her work involves the application of high-trough proteomic and genomic technologies to further our understanding of host-pathogen and immune responses during human infection by V. cholerae (the cause of cholera) and Salmonella enterica serovar Typhi and Paratyphi A (the primary cause of enteric fever) and to identify immunogenic antigens or molecular signatures for vaccine and diagnostic assay development. Research in the lab utilizes human patient samples in addition to using murine models and tumor cell lines to perform a variety of immunological, functional, and genome scale assays. These include but are not limited to ELISAs, flow cytometry, and RNA-sequencing assays to address 1) fundamental questions about protective immunity during human infection with S. Typhi or V. cholerae and the novel mechanisms these pathogens utilize to cause infection and 2) develop rapid diagnostic and seroepidemiological tools that can be used in resource limited settings.
Responsibilities include, but are not limited to, the following activities\:
· Contribute directly to ongoing research projects.
· Lead scientific analysis, abstract submissions, and manuscript development for 1-2 projects at a time.
· Travel to collaborative sites to support our capacity building efforts.
· Supervise/Mentor trainees in the lab.
· Participate in collaborative grant-writing projects.
Duties will be carried out in a typical laboratory environment. Occasional evening or weekend work may be required.
Occasional travel to international research sites may be required. There is also the opportunity to travel to scientific meetings to present research.
The candidate may be responsible for providing guidance and expertise to 1-2 Research Technician and/or students.
*The overall focus of the Charles lab is to decrease the global burden of mucosal and enteric infections of import affecting resource-poor and marginalized populations. More specifically, her work involves the application of high-trough proteomic and genomic technologies to further our understanding of host-pathogen and immune responses during human infection by V. cholerae (the cause of cholera) and Salmonella enterica serovar Typhi and Paratyphi A (the primary cause of enteric fever) and to identify immunogenic antigens or molecular signatures for vaccine and diagnostic assay development. Research in the lab utilizes human patient samples in addition to using murine models and tumor cell lines to perform a variety of immunological, functional, and genome scale assays. These include but are not limited to ELISAs, flow cytometry, and RNA-sequencing assays to address 1) fundamental questions about protective immunity during human infection with S. Typhi or V. cholerae and the novel mechanisms these pathogens utilize to cause infection and 2) develop rapid diagnostic and seroepidemiological tools that can be used in resource limited settings.
Responsibilities include, but are not limited to, the following activities\:
· Contribute directly to ongoing research projects.
· Lead scientific analysis, abstract submissions, and manuscript development for 1-2 projects at a time.
· Travel to collaborative sites to support our capacity building efforts.
· Supervise/Mentor trainees in the lab.
· Participate in collaborative grant-writing projects.
Duties will be carried out in a typical laboratory environment. Occasional evening or weekend work may be required.
Occasional travel to international research sites may be required. There is also the opportunity to travel to scientific meetings to present research.
The candidate may be responsible for providing guidance and expertise to 1-2 Research Technician and/or students.
*Job qualifications include the following\:
· PhD in a life science field
· 7 years of experience in an academic, research, or related setting
· Experience in flow cytometry is required
Additional desired skills\:
Gets Results/Takes Initiative
· High personal work standards and sense of urgency about results
· Ability to anticipate the needs of the group in a fast-paced environment
· Problem-solving ability, including the ability to gather information, identify resources, and imagine alternatives
· Demonstrates initiative and identifies key priorities, even in ambiguous situations with limited direction
· Ability to work effectively under pressure and within short time constraints
· Consistently demonstrates a positive, "can-do" attitude.
· Ability to work successfully in a collaborative environment.
Communication Skills
· Excellent written and verbal communication skills
· Cognizant and respectful of cultural differences in communication approach
This description has been designed to indicate the general nature and level of work performed by an employee within this position. The actual duties, responsibilities and qualifications may vary based on need.
*Job qualifications include the following\:
· PhD in a life science field
· 7 years of experience in an academic, research, or related setting
· Experience in flow cytometry is required
Additional desired skills\:
Gets Results/Takes Initiative
· High personal work standards and sense of urgency about results
· Ability to anticipate the needs of the group in a fast-paced environment
· Problem-solving ability, including the ability to gather information, identify resources, and imagine alternatives
· Demonstrates initiative and identifies key priorities, even in ambiguous situations with limited direction
· Ability to work effectively under pressure and within short time constraints
· Consistently demonstrates a positive, "can-do" attitude.
· Ability to work successfully in a collaborative environment.
Communication Skills
· Excellent written and verbal communication skills
· Cognizant and respectful of cultural differences in communication approach
This description has been designed to indicate the general nature and level of work performed by an employee within this position. The actual duties, responsibilities and qualifications may vary based on need.
*Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged. *Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.