Req #: 240654 Department: DEPARTMENT OF MEDICINE - CARDIOLOGY Posting Date: 10/31/2024 Closing Info: Open Until Filled Salary: $4,276 - $5,020 per month Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701\_a11y.pdf) As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty. **The Department of Medicine - Cardiology has an outstanding opportunity for a Research Scientist Engineer 1 to join their team.** **POSITION PURPOSE** Field of research this position is engaged in: The role of plasma extracellular vesicles in heart failure inflammation. Purpose of the research project(s) this position supports: Recent studies suggest chronic sterile inflammation contributes to heart failure (HF) progression; however, a failure to establish a causal relationship between systemic inflammation and HF outcomes has essentially halted progress in this field. Our lab studies how monocytes, a key player in HF inflammation, amplifies the inflammatory signal via extracellular vesicle. Research Sponsors/Stakeholders: VA, Locke Foundation **DUTIES AND RESPONSIBILITIES** Conducting Experiments – 80% The focus of the Research Scientist Associate position within the Wang Lab is to perform various molecular and cellular biological experiments to produce high quality scientific data for publications, grant applications, and others. More specifically, the scope of the job includes conducting and designing experiments using techniques listed below: (1) Cell-based assays—culturing and stimulate THP-1 cells with various pro-inflammatory stimuli and siRNA knockdown screen (2) ELISA of cytokines of culture media and plasma (3) Measuring EV content in culture media and plasma using FACS (4) Various molecular biological procedures—immunoprecipitation-Western blot analysis, Protein purification, RT-qPCR, immunostaining, and cloning. (5) Writing: (a) Write a protocol and maintain it updated, (b) write methodology and result sections of the manuscripts, (c) generate figures that depict the results of the performed experiments, and (d) re-format the manuscripts for appropriate journal styles. (6) Quantification of scanned or acquired images such as electron microscopic and immunohistochemistry images using image-analysis softwares. The Research Scientist will report to and work closely with the Principal Investigator, Dr. Dennis Wang, who will mentor him/her for his/her professional goals and development. Operations/Administration – 20% + Independently and collaboratively support lab management and operation as part of the research activity, such as: identification and procurement of laboratory supply and service, etc. + Support and participate in regulatory and certification processes and laboratory compliance. + Support and participate in laboratory orientation of new lab personnel. + Collaborate with and support the PI/supervisor with multiple laboratory experiments, as delegated by the PI/supervisor. + Prepare, optimize and execute experiment protocols; analyze the data and generate and present summary and reports for research projects and laboratory activities per instruction. **MINIMUM REQUIREMENTS** + Bachelor’s Degree in Biochemistry, Molecular Biology, Cell Biology, Biotechnology and 1 year of laboratory experience _Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration._ **ADDITIONAL REQUIREMENTS** + Excellent communication skills and good people skills + Self-motivator with positive attitude + Ability to produce the data in a reproducible fashion + Ability to adhere to the established protocols + Ability to improve and trouble-shoot a protocol + Good hands in basic experiments + Experience and significant understanding in molecular biology and/or biotechnology **DESIRED QUALIFICATIONS** + PhD in Biochemistry, Molecular Biology, Cell Biology, Biotechnology + Some real wet lab experience and superior ability to learn the new techniques and protocols. **Application Process:** The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.