Req #: 241562 Department: PHARMACOLOGY Job Location Detail: 100% in person Posting Date: 12/05/2024 Closing Info: Open Until Filled Salary: $4,276 - $4,665 per month Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701\_a11y.pdf) As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty. The Department of Pharmacology and the Molecular Genetics Resource Core within the Center for the Neurobiology of Addiction, Pain, and Emotion has an outstanding opportunity for a Research Scientist Engineer 1 to working with lab researchers to develop Adeno-Associated Viruses for CRISPR/Cas9 mutagenesis and optogenetic studies in mouse models of neuropsychiatric disorders. **DUTIES AND RESPONSIBILITIES** Research Activities 70% + Develop of reagents related to DNA mutagenesis and in the validation of these methods. + Design methodological approaches for manipulating gene expression in the central nervous system of mice. + Analyze data related to DNA mutagenesis. + Assist with general molecular genetics methods in support of ongoing projects. + Assist with documentation for protocols and datasets. + Perform DNA purification and viral vector production.Compliance, and Other Related Duties 30% + Provide lab support for working with institutional animal use and care committee. + Ensuring compliance with Environmental Health and Safety. + Provide assistance with training and onboarding of new personnel. + Procurement of reagents and tracking supplies. + Maintain a clean and sterile working environment. + Maintain equipment and work with facilities or vendors for equipment procurement and repairs. **MINIMUM REQUIREMENTS** + Bachelor’s degree in a related field and one year of experience in pipetting, centrifugation, plasmid purification, and cloning into plasmids **Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.** **ADDITIONAL REQUIREMENTS** + Experience with making basic chemical solutions, + PCR experience, + Experience with cell culturing, + Data management experience + Demonstrated organizational skills **DESIRED QUALIFICATIONS** + Degree in Molecular biology related to plasmid cloning and production. + Experience in PCR for amplification of targeted genes. + Experience in cell culture including cell transfection. + Experience in design of CRISPR/Cas9 guides for gene targeting. + Experience in viral production and purification. **Application Process:** The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.