We are seeking a **_Research Specialist_** that is a driven individual to join a laboratory that uses mouse models of bone marrow transplantation (BMT) to study the epigenetic mechanisms contributing to the development of graft-versus-host disease (GVHD) and the graft-versus-leukemia (GVL) effect. **Specific Duties & Responsibilities** + Conduct murine BMT experiments using established protocols that include, + Harvest of murine spleen for single cell suspension. + Splenic T-cell purificatio. + Splenic T-cell flow cytometry. + Harvest of murine bone marrow. + Animal irradiation. + Tail vein injections. + Participate in long-term monitoring of experiments, including examination of animals and GVHD scoring using an established clinical scale. + Assist with analyzing and interpreting murine GVHD and survival data. + Participate in post-transplant analyses, including the procurement of organs for fixation, and single-cell suspensions for surface and intracellular multiparameter flow cytometry staining of lymphoid and myeloid cells. + Analyze and interpret flow cytometry data. + Collaborate with the PI to oversee scientific management of the laboratory. + Collaborate with PI or other lab members on research projects + Identify and select best options for scientific strategies and research development based on literature reviews and collaboration with colleagues. + Perform a range of laboratory assays. + Contribute to writing of scientific publications and grant applications. + Act as a resource for laboratory techniques for post-doctoral fellow and students and junior technicians. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + One year laboratory experience performing relevant techniques. + Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula. Classified Title: Research Specialist Job Posting Title (Working Title): Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: $17.11 - $29.90 HRLY (Commensurate with experience) Employee group: Full Time Schedule: M-F, 830-500 Exempt Status: Non-Exempt Location: School of Medicine - East Baltimore Campus Department name: 10002936-SOM Onc Pediatric Oncology Personnel area: School of Medicine Equal Opportunity Employer: Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.