We are seeking a **_Research Technologist_** who will follow protocols and procedures to perform general laboratory duties and techniques. 50% of the time will be spent learning biochemical techniques, including cell culture, enzyme activity assays, RT-PCR, western blot, and immunohistochemistry. The remaining 50% of the time will be spent performing animal models of peripheral nerve crush and aging. **Specific Duties & Responsibilities** + Grow and maintain astrocytoma (CCF-STTG1) cells; conduct high-throughput screening of Rapafucin and Life Chemicals’ CNS-focused libraries targeting System xc- in STTG1 cells for glioblastoma and age-related degenerative diseases in the central nervous system. + Assist with evaluating glutamate carboxypeptidase II (GCPII) as a therapeutic target in peripheral nerve degeneration and the function of Schwann cells in aging. + Study efficacy and pharmacokinetic properties of potential treatments using mouse models of peripheral nerve crush and aging. + Maintain aged mouse colonies, including breeding, genotyping, and treating animals. + Assist postdoctoral fellow in behavioral and electrophysiological assessments of aging mouse models. + Assist in performing histological assessments of therapeutic efficacy in peripheral nerve crush and aging mouse models, including tissue collection, sectioning, and immunohistochemical staining. + Perform miscellaneous related duties as assigned. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + Some related post-undergraduate mouse model work experience preferred. Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.50 - $26.00 HRLY ($40,000 targeted; Commensurate with experience) Employee group: Full Time Schedule: M-F, 8:30-5pm Exempt Status: Non-Exempt Location: School of Medicine Campus Department name: ​​​​​​​BSi NeuroTranslational Program Personnel area: School of Medicine Equal Opportunity Employer: Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.