We are seeking a **_Research Technologist_** to work under the general supervision of the assigned faculty investigator, post-doctoral fellow, and lab manager. Semi-independent work, responsible for laboratory research using established procedures and protocols. The research focus of the laboratory is on the dissection of the molecular biology of erythroid leukemogenesis and the discovery of novel combinational therapies to effectively eliminate erythroleukemic cells in chronic and acute myeloid malignancies. Current projects involve in vitro experiments using CRISPR-Cas9 mediated gene editing, CRISPR-Cas9 knockout screens, progenitor, growth and apoptosis assays, flow cytometry, protein extraction, western blotting, and drug screens. Lab projects also involve a significant amount of work with immunocompromised mice (NSG and NSGS) using cell line- and patient-derived MDS and AML xenograft models and transgenic mice. Mouse experiments will involve leukemic cell line and primary sample engraftment, competitive transplantations, blood draws, in vivo imaging, transgenic mice breeding, harvesting, tissue collection, and processing. **Specific Duties & Responsibilities** + Uses molecular biology methods (cloning, PCR assays, gene editing via electroporation), cell culture methods (transfections, transductions, cell line maintenance, drug treatments), biochemical procedures (protein electrophoresis, western blotting, immunoprecipitation, protein purification), flow cytometry analysis, cytotoxicity measurement assays (MTT, apoptosis measurement, clonogenicity assays). + Participates in mice experiments (breeding, leukemic cells engraftment, mice treatment via intraperitoneal and intravenous injections and oral gavage, blood draws, tissue collection, and processing). + Applies analytical skills to implement assigned projects. + Maintains accurate and current data records. + Maintains freezer stock and supply inventory. + Responsible for maintenance of lab and equipment. + Identifies and troubleshoots methods and experimental procedures. + Exercises independent judgment. + Good oral and written communication skills. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. **Preferred Qualifications** + Some related post-undergraduate work experience. Classified Title: Research Technologist Job Posting Title (Working Title): Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.70 - $26.25 HRLY ($21.00/hour targeted; Commensurate with experience) Employee group: Full Time Schedule: M-F 8:30 - 5:00 Exempt Status: Non-Exempt Location: School of Medicine - East Baltimore Campus Department name: 10002935-SOM Onc Hematologic Malignancies Personnel area: School of Medicine Equal Opportunity Employer: Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.