**Join Amgen’s Mission of Serving Patients** At Amgen, if you feel like you’re part of something bigger, it’s because you are. Our shared mission—to serve patients living with serious illnesses—drives all that we do. Since 1980, we’ve helped pioneer the world of biotech in our fight against the world’s toughest diseases. With our focus on four therapeutic areas –Oncology, Inflammation, General Medicine, and Rare Disease– we reach millions of patients each year. As a member of the Amgen team, you’ll help make a lasting impact on the lives of patients as we research, manufacture, and deliver innovative medicines to help people live longer, fuller happier lives. Our award-winning culture is collaborative, innovative, and science based. If you have a passion for challenges and the opportunities that lay within them, you’ll thrive as part of the Amgen team. Join us and transform the lives of patients while transforming your career. Scientist - Cell Line and Assay Development **What you will do** Let’s do this. Let’s change the world. In this vital role you will develop & implement novel bioassays supporting HTS, lead optimization & mechanism of action studies for therapeutic targets + Develop and optimize bioassays to ensure successful implementation and routine execution, supporting high-throughput screening (HTS), hit characterization & assessment, lead optimization, and mechanism-of-action (MOA) studies for integral membrane proteins such as transporters, receptors, and GPCRs. + Design and conduct laboratory experiments, generate and interpret data to facilitate the development of assays and evaluate therapeutic agents. + Learn, apply, and integrate automation solutions during assay development, MOA studies, data analysis, and reporting processes. + Design, optimize, and implement workflows for cell line generation to enable the development of cell-based assay for screening and/or lead optimization. + Plan and execute experiments semi-independently and adapt readily to evolving project needs. + Identify, evaluate, and implement novel methods and techniques to develop robust bioassays, generating reliable and reproducible data efficiently. + Stay current with the latest scientific literature and actively contribute to the interpretation of results. **What we expect of you** We are all different, yet we all use our unique contributions to serve patients. The professional we seek will have these qualifications: **Basic Qualifications:** + Doctorate degree **OR** + Master's degree and 2+ years relevant experience **OR** + Bachelor's degree and 4+ years relevant experience **Preferred Qualifications:** + Experience in collaborative work within a multi-disciplinary team in a biopharmaceutical environment. + Experience in designing and conducting various assays for integral membrane proteins such as transporters, receptors, and GPCRs, using a wide range of in vitro systems, including recombinant proteins, engineered cells, and primary cells with diverse techniques, is highly advantageous. + Strong scientific foundation in understanding and implementing various bioassays and readouts, including TR-FRET, BRET, Alpha, MSD, HCS, luminescence, and fluorescence. + Expertise in the development and implementation of target engagement (TE), activity, functional, and phenotypic, and protein-protein interaction (PPI) assays to assess therapeutic agents, including thermal shift assay (TSA), probe displacement, second messenger, ion flux, reporter, protein stability, PPI (NanoBiT, NanoBRET, etc.), immunoassays, gene activation, and cellular imaging. + Proficiency in cell line engineering utilizing lentivirus-based methods, gene editing techniques (CRISPR), and cutting-edge molecular biology techniques such as the PiggyBac transposon system and the AAVS1 safe harbor targeting system. + Extensive experience in the functional and phenotypic characterization of engineered cells with analytical techniques, including DNA sequencing, RT-qPCR, and Western Blots. + Experience in a variety of bioassays on more physiologically relevant cellular models such as iPSCs, 3D-culture, co-culture, and primary cells. + Competence in setting up and operating multimode plate readers (e.g., PHERAstar, CLARIOstar, FLIPR, EnVision) and automated liquid handlers (e.g., Echo, Dragonfly, Multidrop Combi, Bravo, BioTek Plate Washer, BlueWasher) for the development of 384- and/or 1536-well microtiter plate assays. + Familiarity with software and statistical platforms necessary for analyzing large data sets, including Screener and Spotfire **What you can expect from us** As we work to develop treatments that take care of others, we also work to care for your professional and personal growth and well-being. From our competitive benefits to our collaborative culture, we’ll support your journey every step of the way. The expected annual salary range for this role in the U.S. (excluding Puerto Rico) is posted. Actual salary will vary based on several factors including but not limited to, relevant skills, experience, and qualifications. In addition to the base salary, Amgen offers a Total Rewards Plan, based on eligibility, comprising of health and welfare plans for staff and eligible dependents, financial plans with opportunities to save towards retirement or other goals, work/life balance, and career development opportunities that may include: + A comprehensive employee benefits package, including a Retirement and Savings Plan with generous company contributions, group medical, dental and vision coverage, life and disability insurance, and flexible spending accounts + A discretionary annual bonus program, or for field sales representatives, a sales-based incentive plan + Stock-based long-term incentives + Award-winning time-off plans + Flexible work models, including remote and hybrid work arrangements, where possible **Apply now** **for a career that defies imagination** Objects in your future are closer than they appear. Join us. **careers.amgen.com** In any materials you submit, you may redact or remove age-identifying information such as age, date of birth, or dates of school attendance or graduation. You will not be penalized for redacting or removing this information. **Application deadline** Amgen does not have an application deadline for this position; we will continue accepting applications until we receive a sufficient number or select a candidate for the position. As an organization dedicated to improving the quality of life for people around the world, Amgen fosters an inclusive environment of diverse, ethical, committed and highly accomplished people who respect each other and live the Amgen values to continue advancing science to serve patients. Together, we compete in the fight against serious disease. Amgen is an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability status, or any other basis protected by applicable law. We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation. Amgen is an Equal Opportunity employer and will consider you without regard to your race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status. We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation.