REQ#: RQ195938Public Trust: NACI (T1) Requisition Type: Regular Your Impact

Own your opportunity to work alongside federal civilian agencies. Make an impact by providing services that help the government ensure the well being of U.S. citizens.

Job DescriptionGDIT is seeking a Senior Research Technician so support the Walter Reed Army Institute of Research (WRAIR) Entomology Branch’s support to the Walter Reed Biosystematics Unit (WRBU). Specific emphasis is on the development of morphological keys and molecular identification assays that permit accurate identifications in austere environments. The goal is to facilitate front facing platforms to support near real-time accurate field identifications of vector and pathogens, to support accurate and timely production of vector-borne disease risk assessments.

HOW YOU WILL MAKE AN IMPACT :
 Develop, validate and implement molecular assays for the rapid, accurate detection and identification of multiple vector and pathogen targets using multiple forward facing platforms (e.g. metabarcoding, MinION, MAGPIX).Develop standard operating procedures to formalize testing procedures using these assays so that multiple testing entities may produce comparable data suitable for use in surveillance programs.Support RDT&E activities for the development of accurate vector identification tools for use by the warfighter. Objectives include high-quality, peer-reviewed scientific publications, the development of unique DNA barcode reference libraries / whole genome sequences for disease vectors, accession of DNA barcodes to the BOLD (Barcode of Life) database.Upload a report of gene/genome sequences generated, and barcode reference sequences uploaded to the BOLD database shall be provided as part of the Monthly Progress ReportProduction of multiplex identification assays for pathogens and vectors (e.g. MAGPIX, PCR Assays, metabarcoding, MinION), the development of new taxonomic LUCID keys and updates of these keys to stand-alone Apps, and taxonomic research on vectors of biomedical importance.
Requirements:B.S with 5 years of experience or, M.S with 2 years of experience preferred; in molecular biology, biology, or biochemistry or related field.Strong publication and research grant award historyMastery of high throughput DNA/RNA extraction techniques, PCR, Sanger sequencing, next generation sequencing: expertise in primer and/or probe development for multiplex / targeted molecular assays, is essential.Proven experience of designing novel multiplex pathogen detection assays using PCR MAGPIX, MinION is essentialWorking knowledge of RT-PCR, qPCR, LAMP, immunological assays (such as ELISA, Western Blot, etc) is desirable.Strong scientific communication skills are required, as applicant shall be expected to prepare SOPs, scientific manuscripts and deliver oral presentations.Ability to work in laboratory and field environments; may require working evenings and weekends; may require travel and ability to work independently following a brief period of specific technical training.
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