As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better. Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability. Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive. **Job Location (Full Address):** 601 Elmwood Ave, Rochester, New York, United States of America, 14642 **Opening:** Worker Subtype: Regular Time Type: Full time Scheduled Weekly Hours: 40 Department: 400094 Neurology - NMD M & D Work Shift: UR - Day (United States of America) Range: UR URG 109 Compensation Range: $55,955.00 - $78,336.00 _The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations._ **Responsibilities:** A position for Technical Associate II is open in the laboratory of the Wellstone Muscular Dystrophy Research Center, an NIH-designated center of excellence. This laboratory, directed by Dr. Charles Thornton, is recognized as a world leader for studies of genetic diseases and therapeutic development. Typical Duties: - Perform analysis of expanded repeats and other genetic variants in DNA. This includes isolation or amplification of DNA segments for measurement of repeat size by several methods, including capillary and gel electrophoresis, Southern blotting, and long-read single-molecule sequencing. The latter methods use several different sequencing platforms, and will require development of new methods and optimization of methods that currently exist. Also, high-throughput sequencing will be used to analyze single-nucleotide genetic variants. Results will be reviewed for quality control metrics and then organized for analysis by bioinformatic experts. Datasets and results will need to be generated for manuscript preparation and grant applications. 25% - Analysis of RNA for alternative splicing and gene expression. This includes extracting RNA from tissue samples, synthesis of cDNA, and then preparation of libraries for targeted RNA sequencing. Including in this task is maintenance of biobanks and inventories of tissue samples, customization of procedures for preparing libraries, review of quality control metrics before submission to sequencing centers, interactions with staff at the sequencing centers (UR and elsewhere) to provide instructions for sequencing runs, review of results for sequencing efficiency, and interface with bioinformatics experts who perform subsequent analyses. Maintaining lab notebooks and sample logs for all experiments is a key activity. - Following approved protocols for the conduct of research, develops and installs laboratory operating procedures. Develops and modifies test methods, selects procedures for data collection and handling, and develops programs for conversions and statistical treatment of data. Analyze and evaluate experimental data and interpret results; assesses the importance of findings in relation to the general research program. Prepare written reports and assist in writing of the principal project report as well as the writing of articles for publication. - Perform other molecular biology protocols, such as, Western blots, DNA cloning, cell transfection. Although managing mouse colonies is not a core responsibility, cross-training for mouse research is desirable to accommodate peak loads and cross-coverage with other people in the lab. Experience with operation of a cryostat and preparation/processing of frozen sections for histologic analysis and in situ hybridization is desirable but not essential. - Training and oversight of undergraduate students and other lab staff working on experiments. Assist with training of graduate students and post-doctoral fellows. Counsel them on technical problems, advise and assist them with special research projects. Insures that health and safety practices are maintained in accordance with University policy and the law. - Assist with lab maintenance tasks including maintenance of laboratory space/reagents and writing protocols. Maintains inventory and equipment as needed and as directed by laboratory manager. Utilizes research literature to assist with developing new methods and experimental design. Requirements: - Bachelor's degree with course work in field of assignment, and at least 2 years of related work experience involving direct assistance to researchers; or any equivalent combination of experience, training, and education. At least 5 years of experience in research preferred. - Must have strong attention to detail and have excellent interpersonal and communication skills with faculty, technical staff, postdocs, graduate students, undergraduate students and other labs. - Must be highly organized, self-motivated individual capable of working independently in a collaborative setting. **EOE Minorities / Females / Protected Veterans / Disabled:** The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University’s mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law. This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations. Notice: If you are a **Current** **Employee,** please **log into myURHR** to search for and apply to jobs using the Jobs Hub. Your application, if submitted using this portal, cannot be moved forward. **Learn. Discover. Heal. Create.** Located in western New York, Rochester is our namesake and our home. One of the world’s leading research universities, Rochester has a long tradition of breaking boundaries—always pushing and questioning, learning and unlearning. We transform ideas into enterprises that create value and make the world ever better. If you’re looking for a career in higher education or health care, the University of Rochester may offer the perfect opportunity for your background and goals At the University of Rochester, we commit to diversity, equity, and inclusion and united by a strong commitment to be ever better—Meliora. It is an ideal that informs our shared mission to ensure all members of our community feel safe, respected, included, and valued.