Adecco Healthcare & Life Sciences is hiring a contract Scientist for our Pharmaceutical partner in Waltham, MA. The anticipated hourly wage for this position is between $56 and $57.62. Hourly wage may depend upon experience, education, geographic location, and other factors. Benefit offerings include medical, dental, vision, life insurance, short-term disability, additional voluntary benefits, EAP program, commuter benefits and 401K plan. Our program provides employees the flexibility to choose the type of coverage that meets their individual needs. Available paid leave may include Paid Sick Leave, where required by law; any other paid leave required by Federal, State, or local law; and Holiday pay upon meeting eligibility criteria Description: Scientist, Liver gene therapy mechanism of action, Genomic Medicine Unit **Basic Qualifications** · Demonstrated expertise with standard aseptic cell culture technique, including experience culturing various primary cells. Experience with hepatocytes primary cell culture is highly appreciated. Experience with hepatocytes and/or liver non-parenchymal cells isolation is a plus. · Broad experience with cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays. Experience with co-culture in-vitro or MPS is a plus. · Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging. Experience with high content microscopy is a plus. · Broad scientific knowledge in liver biology and non-viral delivery and molecular biology. · Self-motivated with strong time management and organizational skills · Flexibility to rapidly changing priorities and deadlines · Experience in preparing high quality documents including technical reports, presentations, SOPs, and experimental protocols. · Highly collaborative with demonstrated ability to work in a matrixed team environment. · Excellent oral and written communication skills **Key Responsibilities:** · Maintain cultures of immortalized tumor cell lines, hepatic primary cell types (e.g. hepatocytes, LSEC and KC). · Perform hepatocytes mouse isolation from key disease models. · Optimize, design, execute, and interpret in vitro cell-based assays to characterize diverse mechanisms of gene therapies (e.g. ELISA, MSD, high content imaging) · Perform fit-for-purpose in vitro and ex vivo assays to assess phenotypic and functional read-outs to assess drug candidate toxicity and efficacy. · Prepare experimental protocols, analyze experimental data, and present study results in team and department meetings. · Document experimental methods and findings in electronic laboratory notebook **Position Objectives:** · Conduct and interpret cell-based in vitro assays to characterize mechanism of action related to non-viral gene therapy with focus on the hepatic environment: · Immunomodulation · Cargo cell localization · Support non-viral (LNP) oligo therapy screenings e.g. endosomal scape assessment **Additional Comments:** Title: Scientist, Liver gene therapy mechanism of action, Genomic Medicine Unit Broad scientific knowledge in liver biology and non-viral delivery and molecular biology. Experience with standard aseptic cell culture technique, including experience culturing various primary cells, hepatocytes primary cell culture, hepatocytes and/or liver non-parenchymal cells isolation. Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging. Experience with high content microscopy is a plus. Cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays. Experience with co-culture in-vitro or MPS is a plus. **Pay Details:** $56.00 to $57.62 per hour Benefit offerings available for our associates include medical, dental, vision, life insurance, short-term disability, additional voluntary benefits, EAP program, commuter benefits and a 401K plan. Our benefit offerings provide employees the flexibility to choose the type of coverage that meets their individual needs. In addition, our associates may be eligible for paid leave including Paid Sick Leave or any other paid leave required by Federal, State, or local law, as well as Holiday pay where applicable. Equal Opportunity Employer/Veterans/Disabled To read our Candidate Privacy Information Statement, which explains how we will use your information, please navigate to https://www.adecco.com/en-us/candidate-privacy The Company will consider qualified applicants with arrest and conviction records in accordance with federal, state, and local laws and/or security clearance requirements, including, as applicable: + The California Fair Chance Act + Los Angeles City Fair Chance Ordinance + Los Angeles County Fair Chance Ordinance for Employers + San Francisco Fair Chance Ordinance